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A New Method for Objective Analysis of Detrusor Rhythm during the Filling Phase
Ashley B King1, Adam Klausner1, Samuel Robinson1, David Rapp2, Vikram Sabarwal1, John Speich1, Harry Koo1, Paul Ratz1
1Virginia Commonwealth University, Richmond, VA;2Virginia Urology Center for Incontinence and Pelvic Floor Reconstruction, Richmond, VA

Introduction: A standardized model for quantitative analysis of detrusor rhythmic contraction currently does not exist. The goal of this study was to develop a computer program for analyzing detrusor rhythm.
Materials & Methods: Seventeen detrusor strips from 12 rats of 3 different strains (Wistar, WKY, and SHR) were used to analyze rhythm. At optimal length, prostaglandin-E2 (PGE2) was added in half-log increments from 1.0x10-9M to 1.0x10-6M. Then maximum and minimum force values were obtained using KCl and Ca2+-free solution containing EGTA, respectively. A computer program was developed using DASYLab 10.0 to analyze the effects of PGE2 on frequency (contractions/5min), amplitude (5min area under the curve), and tone (5min average) in a step-wise fashion shown in Figure 1. The computer generated frequency count was compared to human assessment.
Results: PGE2 induced a concentration-dependent increase in frequency, amplitude, and tone. These effects were documented in a reproducible, consistent way using the computer program, and the frequency count was significantly different from human assessment.
Conclusions: A computer program for rhythm analysis was developed and tested using detrusor strips of rats from different genders and strains. The program analyzed detrusor rhythm in terms of frequency, amplitude, and contractile tone in an Objective and reproducible manner. Further testing may allow this program to compare the effects of different agents on rhythmic activity during the filling phase.


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